Pathogens prevalence in acute gastroenteritis

Prevalence of bacterial, viral, and parasitic infection in acute gastroenteritis

Acute gastroenteritis is a common infectious disease which causes nausea, vomiting, diarrhoea, and abdominal pain usually lasting fewer than 14 days.¹Annually, there are an estimated 21 million cases in France and 17 million cases in the UK.^2,3 While worldwide, it affects between 3 and 5 million children and accounts for between 1.5 and 2.5 million deaths annually.⁴ Viruses are the culprits in 50-70% of cases followed by bacteria (15-20%) and parasites (10-15%) (Table 1).

Table 1. Infectious causes of acute gastroenteritis¹

Note: Pathogens are listed in order of prevalence

The precise incidence and cause of acute infectious gastroenteritis is difficult to assess as not all infected individuals report their symptoms or seek medical care. Additionally, stool cultures used to identify bacterial causes of gastroenteritis only detect pathogens in 1.5% to 5.6% of cases, making it more difficult to accurately track and appropriately treat patients.¹

Most common bacterial causes

1. C. jejuni and C. coli are established causes of clinically indistinguishable diarrhoea in humans. Infection with either can occur in patients of all ages, with evidence that it is more prevalent in the 1–4 and 15–24-year age groups.⁵ There has been a rise in global incidence of campylobacteriosis in the past decade, even though cases of C. jejuni and C. coli are likely to be underreported. Although C. coli is less prevalent than C. jejuni in many geographic regions, it can contribute as many as 25% of all gastroenteritis cases caused by Campylobacter spp.⁵

Nontyphoidal Salmonella has a high impact on global human health, causing an estimated 93.8 million illnesses (approximately 3% of diarrhoeal illnesses worldwide) – 80.3 million of which are foodborne – and 155,000 deaths each year.⁶

Most of the disease burden of global infection with Shigella spp is borne by children aged 1–4 years, living in low-income and middle-income settings.⁷ Although mortality rates have fallen over the past 30 years, approximately 164,000 annual deaths are attributable to shigellosis.⁷

Most common viral causes

Norovirus is the most common cause of acute gastroenteritis (one in five cases), causing an estimated 685 million cases annually.⁸ Two hundred million of these are in children under the age of five years. Although norovirus leads to an estimated 50,000 child deaths every year, mostly in developing countries, it is not restricted to low-income countries and is frequently the source of outbreaks in close quarters (e.g., cruise ships)^8–10. Norovirus infections result in an estimated $60 billion annually due to healthcare costs and lost productivity.⁸

Rotaviruses are ubiquitous, infecting almost every child globally by 3–5 years of age and are a leading cause of severe, dehydrating gastroenteritis. In 2013, they were associated with an estimated >200,000 fatalities in children <5 years old globally.¹¹ The prevalence of rotavirus infection in children hospitalised with diarrhoea is approximately 30 –50%, however >90% of children with fatal rotavirus infections live in low-income countries.¹¹ Countries that have implemented rotavirus vaccination programmes have had a notable decline in the number of acute gastroenteritis cases, particularly in countries with low child mortality, among younger age groups, and in countries with higher coverage.¹²

Enteric adenovirus types 40 and 41 are one of the most important agents of acute gastroenteritis among infants and young children less than 2 years of age, with rates varying from 1–8% in developed countries, to 2–31% in developing countries.⁴

Most common parasitic causes

Giardia lamblia (also referred to as Giardia intestinalis and Giardia duodenalis) is the most common intestinal parasite in the world, affecting approximately 200 million people in Asia, Africa, and Latin America.¹³

Another common parasitic cause of infectious diarrhoea is Entamoeba histolytica, which affects 50 million people annually.¹⁴ This pathogen shares morphological similarities with Entamoeba dispar, a typically non-pathogenic parasite.¹⁵ Due to their similarities in appearance, microscopy cannot differentiate between the 2 parasites, which can lead to false-positive results.¹⁵

Molecular testing for gastroenteritis

Conventional culture tests are still the routine testing methods for the diagnosis of bacterial enteropathogens and pathogens detected from culture – testing can be sent for further identification, outbreak investigations, and epidemiological studies.¹⁶ Although the specificity of culture testing is 100% if no pathogens are present in healthy specimens, the sensitivity of these tests are usually low, difficult to determine, and requires 3–5 days for pathogen detection and finalizing reports. ¹⁶ They also require highly trained staff, especially for parasite microscopy, and specific equipment to detect some of the pathogens in the clinical specimens.¹⁶

Antigen detection is another popular testing method to detect certain intestinal parasites and viruses. ¹⁶ They can be done using commercially available immunoassays. However, they do generally have low sensitivity and do not detect all the pathogens involved in gastrointestinal infections.¹⁶

The emergence of commercially available nucleic acid amplification tests (NAATs) has allowed for easier detection of pathogens.¹⁶ Common single molecular assays are used for the detection of many pathogens that cause gastroenteritis.¹⁶These assays can target specific patient populations and can be simultaneously tested using multiplex platforms, which have multiple testing panels. To exploit this feature, a targeted syndromic approach can be used in which multiple pathogens associated with a particular syndrome can be tested at once, saving time and costs and providing better patient management by enabling earlier initiation of treatment.¹⁷

NAAT-based technologies have helped overcome some of the challenges that arise from traditional microbiological and culture methods.¹⁶The high throughput, sensitivity and specificity of molecular-based testing provided by NAATs allow rapid diagnosis, treatment and management of gastrointestinal infections.¹⁶The COVID-19 pandemic revealed what was lacking in diagnostic testing, bringing about a greater awareness of molecular diagnostics. These technologies have great promise for non-COVID-testing and can deliver test results faster, potentially leading to earlier diagnosis and intervention and more rapid assessments of trends in incidence.¹⁸

References

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